Olive biosorbent

Olive biosorbent (OB): Olive tree legs and green leaves were cut to small particle size then washing with water, drying at 250 ?C and blending using food blender. Carboxy olive biosorbent (COB) preparation: 100 g of OB with 80 mL of concentrated HNO3 were heating in a beaker until brown foams finished, then washing with distilled water and methanol then drier at 80 ?C.
Olive/oleic bio-alkyd resin (OOBAR) Preparation: A 85 g of oleic acid with 28 g of glycerol were heated for 6 h. Then the product was heated with 15 g biosorbent 6 h, followed by adding 15 g of phthalic anhydride then heated 6 h. The final product was washed with distilled water then methanol and left to dry at room temperature then blending and sifting at 355 µm (0.0355 cm).
Molybdenum stock solution (1 g/L) preparation: 0.185 g of (NH4)6Mo7O24.4H2O was dissolved in distilled water to 100 mL.
Vitamins drugs stock solutions: 1.217 g Vitayami for the deficiency of iron and vitamins tablets which contain (Cu: 1000 mcg; Fe: 30 mg: Mn: 2.5 mg: Mo: 15 mcg) and other content (Multi-Apex for Pharmaceutical Industries, Badr City – Cairo – Egypt), 1.067 g V2 plus multivitamins and minerals capsules which contain (Mo: 0.2 mg; Cu: 1 mg: Mn: 1 mg: Fe: 10 mg) and other content (Pharco pharmaceuticals, Alexandria – Egypt), 1.299 g Vitamax plus dietary supplements capsules which contain (Cu: 2 mg: Fe: 9 mg; Mn: 5 mg; Mo: 30 mcg) and other content (El Salam City – Cairo – Egypt) and 1.67 g Vitona plus energized and biotonic capsules which contain (Fe: 14 mg; Cu: 2 mg; Mn: 2.5 mg; Mo: 0.186 mg) and other contents (Egyptian Int. Pharmaceutical Industries CO, E. I. P. CO, 10th of Ramadan City – Egypt) were prepared by dissolving of each one in aqua regia and gently evaporated several times till dryness and removing any excess of them. The residual was dissolved in distilled water to 100 mL in a measuring flask containing 1mL of concentrated HNO3.
The tissue of lever mice stock solution: A 0.5 g of liver tissue spiked with Mo nanoparticle (0.25 mg) was prepared by dissolving in aqua regia and gently evaporated several times till dryness and removing any excess of them. The residual was dissolved in distilled water to 10 mL in a measuring flask containing 1mL of concentrated HNO3.
2.3. Recommended procedures
A 0.1 g of OOBAR was mixed with adjust concentration of molybdenum solution, H2SO4, L-ascorbic acid, and NH4SCN then diluted to 25 mL and shaken 60 min at room temperature. The remaining concentrations of Mo(V) were determined using spectrophotometrically (?max 485 nm) 41. The sorption percentage of Mo(V) and sorption capacity of OOBAR (Q, mmol/g) were calculated.
By using a dynamic technique, 10 g of OOBAR was packed through glass column which has 35 cm long and 1.5 cm in diameter with a bed height at L= 15 cm. A series of 25 mL of tap water, liver mice tissue or vitamins solutions (n = 5) were passed through the OOBAR columns at different flow rate 0.2-1.7 mL/min. The effluent solutions were collected and analyzed spectrophotometrically. Mo(V) was eluted from OOBAR columns with NH4OH (0.05 mol/L) as eluent at a flow rate of 3 mL/min then determined spectrophotometrically